PGKp-GFP-YAP
(Plasmid #174172)

• Purpose: Lentiviral vector to constitutively express YAP under control of the human PGK promoter.
• Depositing Lab: Rod Bremner
• Publication: Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 10.1016/j.ccell.2021.06.016.

Backbone

• Vector backbone: pLKO.1
• Backbone size w/o insert (bp): 8100
• Total vector size (bp): 9660
• Modifications to backbone: U6-shRNA sequence was removed and the original puromycin resistance gene was replaced with an IRES-GFP-T2A-puroR sequence.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Recommend TB over LB media.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YAP
• Alt name: YAP1
• Species: H. sapiens (human)
• Insert Size (bp): 1584
• Promoter: PGK
• Tag / Fusion Protein:
  • 3x FLAG tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CAATAGCGGCTGCTCAG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jeff Wrana

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry