C152
(Plasmid
#174355)
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PurposeRepair plasmid for introducing AID, T2A and mCherry at the C-teminus of human PABPC1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174355 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneUnknown
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAuxin-inducible degron (AID) and mCherry
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
AID, T2A and mCherry are flanked by homology arms of human PABPC1 genomic regions near the stop codon. Used with C119 and the PAM region is mutated.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C152 was a gift from David Bartel (Addgene plasmid # 174355 ; http://n2t.net/addgene:174355 ; RRID:Addgene_174355) -
For your References section:
The molecular basis of coupling between poly(A)-tail length and translational efficiency. Xiang K, Bartel DP. Elife. 2021 Jul 2;10. pii: 66493. doi: 10.7554/eLife.66493. 10.7554/eLife.66493 PubMed 34213414
