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The molecular basis of coupling between poly(A)-tail length and translational efficiency.
Xiang K, Bartel DP
Elife. 2021 Jul 2;10. pii: 66493. doi: 10.7554/eLife.66493. PubMed Article

Plasmids from Article

ID Plasmid Purpose
174337C022Template for making Rluc mRNA reporter with no pA.
174338C031Template for making Rluc mRNA reporter with pA30.
174339C033Template for making Rluc mRNA reporter with pA63.
174340C034Template for making Rluc mRNA reporter with pA98.
174341C035Template for making Rluc mRNA reporter with pA120.
174342C054Template for making Fluc mRNA reporter with pA124 and mutant mouse Malat1.
174343C071Template for making Nluc mRNA reporter with pA29.
174344C093Template for making Nluc mRNA reporter with pA63.
174345C098Template for making Nluc mRNA reporter with pA139.
174346C077Template for making mRNA for overexpressing frog PABPC1 (C-FLAG) in frog oocytes
174347C078Template for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytes
174348C079Template for making mRNA for overexpressing eGFP in frog oocytes
174349C233Template for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytes
174350C150Template for making mRNA for overexpressing human eIF4G (C-FLAG) in frog oocytes
174351C245Template for making mRNA for overexpressing frog RPL3 (C-HA) in frog oocytes
174352C255Template for making mRNA for overexpressing eGFP (C-FLAG+HA) in frog oocytes
174353C064Plasmid for expressing frog PABPC1 (C-His) in E.coli
174354C119CRISPR-Cas9 with guide targeting human PABPC1 genomic location near STOP codon
174355C152Repair plasmid for introducing AID, T2A and mCherry at the C-teminus of human PABPC1
174356C278For introducing multiple copies of OsTIR1 with the piggyBac system
174357C194For expressing GFP in human cell lines via transfection
174358C204For expressing siRNA-resistant human PABPC1 in human cell lines via transfection
174359C205For expressing siRNA-resistant frog ePAB in human cell lines via transfection
174360C211For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection

Antibodies from Article