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PylT(CTAG)WT PylS mCherry-P2A-eGFP(CTAG)
(Plasmid #174527)

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Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174527 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 174527-D50 50 μg of DNA in Tris buffer $495
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DNA 174527-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    PB53
  • Total vector size (bp) 10600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PylS
  • Alt name
    MmPylRS
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    1395
  • GenBank ID
    NC_003901.1
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PylT(CTAG)WT PylS mCherry-P2A-eGFP(CTAG) was a gift from Yu-Hsuan Tsai (Addgene plasmid # 174527 ; http://n2t.net/addgene:174527 ; RRID:Addgene_174527)

  • For your References section:

    Development of mammalian cell logic gates controlled by unnatural amino acids. Mills EM, Barlow VL, Jones AT, Tsai YH. Cell Rep Methods. 2021 Sep 16;1(6):100073. doi: 10.1016/j.crmeth.2021.100073. eCollection 2021 Oct 25. 10.1016/j.crmeth.2021.100073 PubMed 35474893
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