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pB80-hKif5b(1-560)rigor-2xmNeongreen
(Plasmid #174649)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174649 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 174649-D50 50 μg of DNA in Tris buffer $495
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DNA 174649-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    B-actin
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hKif5B(1-560)rigor-2xmNeongreen
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3156
  • Mutation
    rigor mutation G234A
  • Entrez Gene
    KIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
  • Promoter Chicken beta-actin
  • Tag / Fusion Protein
    • mNeongreen-mNeongreen (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal plasmid ID: KJ116

Please visit https://www.biorxiv.org/content/10.1101/2021.06.23.449589v1 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB80-hKif5b(1-560)rigor-2xmNeongreen was a gift from Lukas Kapitein (Addgene plasmid # 174649 ; http://n2t.net/addgene:174649 ; RRID:Addgene_174649)

  • For your References section:

    A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle. Jansen KI, Iwanski MK, Burute M, Kapitein LC. J Cell Biol. 2023 May 1;222(5):e202106105. doi: 10.1083/jcb.202106105. Epub 2023 Mar 2. 10.1083/jcb.202106105 PubMed 36880745
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