pLenti CMV Puro LUC (w168-1) Notes
Addgene diagnostic digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: ClaI and EcoRI
We expected the following band sizes for this digest: 9.6kb for ClaI and 7.3kb + 2.3kb for EcoRI. We see the expected pattern.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid pLenti CMV Puro LUC (w168-1) with the following enzymes: NcoI-HF and PvuII-HF.
NcoI-HF: 5.2 kb, 2.1 kb, 2 kb, & 0.3 kb
PvuII-HF: 6.3 kb, 2.5 kb, and 0.8 kb
We observe the expected bands for this digest.