pET16better_H10_HRV3C_chitinase
(Plasmid
#175091)
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PurposeExpresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175091 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-16b
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Backbone manufacturerNovagen (EMD Millipore)
- Backbone size w/o insert (bp) 5651
- Total vector size (bp) 7442
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Modifications to backboneFactor Xa cleavage site replaced by HRV-3C cleavage site
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namechitinase
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SpeciesB. licheniformis
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Insert Size (bp)1866
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Tag
/ Fusion Protein
- 10X His, HRV-3C (N terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET16better_H10_HRV3C_chitinase was a gift from Jay C Dunlap (Addgene plasmid # 175091 ; http://n2t.net/addgene:175091 ; RRID:Addgene_175091) -
For your References section:
Quantitative single molecule RNA-FISH and RNase-free cell wall digestion in Neurospora crassa. Bartholomai BM, Gladfelter AS, Loros JJ, Dunlap JC. Fungal Genet Biol. 2021 Aug 20;156:103615. doi: 10.1016/j.fgb.2021.103615. 10.1016/j.fgb.2021.103615 PubMed 34425213
Map uploaded by the depositor.
