pCDNA4TO-3XFLAG Halo WT nsp1
(Plasmid #175412)

• Purpose: Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.
• Depositing Lab: Britt Glaunsinger
• Publication: The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression. (unpublished)

Backbone

• Vector backbone: pCDNA4
  (Search Vector Database)
• Backbone size w/o insert (bp): 6028
• Total vector size (bp): 6567
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CoV2 nsp1
• Alt name: nsp1
• Species: CoV2
• Insert Size (bp): 539
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3XFLAG Halo (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry