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The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression.
Mendez AS, Ly M, Gonzalez-Sanchez AM, Hartenian E, Ingolia NT, Cate JH, Glaunsinger BA
Cell Rep. 2021 Oct 19;37(3):109841. doi: 10.1016/j.celrep.2021.109841. Epub 2021 Sep 30.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
175412pCDNA4TO-3XFLAG Halo WT nsp1Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.
175417pCDNA4TO-3XFLAG Halo R124A/K125A nsp1 Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.
175421pCDNA4TO-3XFLAG Halo K164A/H165A nsp1Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.
175422pCDNA4TO-3XFLAG Halo R99A nsp1Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.
175423pCDNA4TO-3XFLAG Halo delta 1-117 nsp1Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.
175424pCDNA4 3xFLAG Halo delta 118-180 nsp1Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.
175425pCDNA4 3xFLAG Halo delta 122-130 nsp1Expresses delta 122-130 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.
175426pCDNA4 3xFLAG Halo G-Linker nsp1Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.
175427pCDNA4 3xFLAG Halo E55A E57A K58A nsp1Expresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.
175428pCDNA4 3xFLAG Halo R119A K120A nsp1Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.
175429pCDNA4 3xFLAG Halo E36A E37A nsp1Expresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.
175430pJP-CoV2leader-nLuc-TSSExpresses nLuc with 5' leader of CoV2 in mammalian cells.
175431pJP-HBB-nLucExpresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.
175432pUC57-HBB-nLucUsed to generate IVT RNA for translation extracts
175512pGEX nsp1 CoV2For protein expression of WT nsp1 CoV2
175513pGEX nsp1 R124A/K125A CoV2For protein expression of R124A/K125A nsp1 CoV2
175514pGEX nsp1 K164A/H165A CoV2For protein expression of K164A/H165A nsp1 CoV2
175515pGEX nsp1 R99A CoV2For protein expression of R99A nsp1 CoV2
175516pGEX C_K nsp1 CoV2Expresses C_K for bioconjugation on nsp1
175517pGEX C_K nsp1 K164A/H165A CoV2Expresses C_K for bioconjugation on nsp1
175518pUC Ad VAIFor mammalian expression of adenovirus VAI RNA
176057pCDNA4 CoV-2 nsp1 3xFLAGExpressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.
176059pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAGExpressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.
176060pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAGExpresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.
176061pCDNA4TO R99A CoV-2 nsp1 3xFLAGExpresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

Antibodies from Article