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Addgene

pCDNA4 3xFLAG Halo delta 122-130 nsp1
(Plasmid #175425)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 175425 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDNA4
  • Backbone size w/o insert (bp) 6028
  • Total vector size (bp) 6540
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CoV2 delta 122-130 nsp1
  • Alt name
    nsp1 RNase destabilization deletion mutant
  • Species
    CoV2
  • Insert Size (bp)
    512
  • Promoter CMV
  • Tag / Fusion Protein
    • 3XFLAG Halo (N terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA4 3xFLAG Halo delta 122-130 nsp1 was a gift from Britt Glaunsinger (Addgene plasmid # 175425 ; http://n2t.net/addgene:175425 ; RRID:Addgene_175425)
  • For your References section:

    The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression. Mendez AS, Ly M, Gonzalez-Sanchez AM, Hartenian E, Ingolia NT, Cate JH, Glaunsinger BA. Cell Rep. 2021 Oct 19;37(3):109841. doi: 10.1016/j.celrep.2021.109841. Epub 2021 Sep 30. 10.1016/j.celrep.2021.109841 PubMed 34624207