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Addgene

pCDNA4TO R99A CoV-2 nsp1 3xFLAG
(Plasmid #176061)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 176061 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDNA4TO
  • Backbone size w/o insert (bp) 5163
  • Total vector size (bp) 540
  • Vector type
    Mammalian Expression
  • Selectable markers
    Bleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CoV-2 nsp1
  • Alt name
    R99A N-terminal mutant
  • Species
    SARS-CoV-2
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA4TO R99A CoV-2 nsp1 3xFLAG was a gift from Britt Glaunsinger (Addgene plasmid # 176061 ; http://n2t.net/addgene:176061 ; RRID:Addgene_176061)
  • For your References section:

    The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression. Mendez AS, Ly M, Gonzalez-Sanchez AM, Hartenian E, Ingolia NT, Cate JH, Glaunsinger BA. Cell Rep. 2021 Oct 19;37(3):109841. doi: 10.1016/j.celrep.2021.109841. Epub 2021 Sep 30. 10.1016/j.celrep.2021.109841 PubMed 34624207