pGEX C_K nsp1 CoV2
(Plasmid
#175516)
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PurposeExpresses C_K for bioconjugation on nsp1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175516 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX
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Backbone manufacturerGE (cytiva)
- Backbone size w/o insert (bp) 4950
- Total vector size (bp) 5490
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameC_K nsp1 CoV2
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Alt nameWT nsp1 for bioconjugation
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SpeciesCoV2
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Insert Size (bp)540
- Promoter tac promoter
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Tag
/ Fusion Protein
- GST tag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEX forward
- 3′ sequencing primer pGEX reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX C_K nsp1 CoV2 was a gift from Britt Glaunsinger (Addgene plasmid # 175516 ; http://n2t.net/addgene:175516 ; RRID:Addgene_175516)