pUC57-HBB-nLuc
(Plasmid
#175432)
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PurposeUsed to generate IVT RNA for translation extracts
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175432 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC57
- Backbone size w/o insert (bp) 2257
- Total vector size (bp) 2839
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Vector typeBacterial Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHBB-nLuc
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Alt nameHBB-nLuc (IVT)
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SpeciesSynthetic
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Insert Size (bp)588
- Promoter T7
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC57-HBB-nLuc was a gift from Britt Glaunsinger (Addgene plasmid # 175432 ; http://n2t.net/addgene:175432 ; RRID:Addgene_175432)