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Addgene

pUC57-HBB-nLuc
(Plasmid #175432)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175432 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 2257
  • Total vector size (bp) 2839
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HBB-nLuc
  • Alt name
    HBB-nLuc (IVT)
  • Species
    Synthetic
  • Insert Size (bp)
    588
  • Promoter T7

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-HBB-nLuc was a gift from Britt Glaunsinger (Addgene plasmid # 175432 ; http://n2t.net/addgene:175432 ; RRID:Addgene_175432)

  • For your References section:

    The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression. Mendez AS, Ly M, Gonzalez-Sanchez AM, Hartenian E, Ingolia NT, Cate JH, Glaunsinger BA. Cell Rep. 2021 Oct 19;37(3):109841. doi: 10.1016/j.celrep.2021.109841. Epub 2021 Sep 30. 10.1016/j.celrep.2021.109841 PubMed 34624207
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