PRDX6A-c001
(Plasmid #175474)

• Purpose: Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.
• Depositing Lab: Opher Gileadi
• Publication: TREAT-AD plasmids 2021 (unpublished)

Backbone

• Vector backbone: pNIC-Bio3
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PRDX6
• Alt name: 1-Cys|AOP2|HEL-S-128m|NSGPx|PRX|aiPLA2|p29
• Species: H. sapiens (human)
• Mutation: See Depositor Comments Below
• Entrez Gene: PRDX6 (a.k.a. 1-Cys, AOP2, HEL-S-128m, LPCAT-5, NSGPx, PRX, aiPLA2, p29)
• Tags / Fusion Proteins:
  • His6-TEV (N terminal on insert)
  • AviTag (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2-pBirA cells for expression.

Please note: Plasmid contains an A111T mutation in PRDX6. It is not known if this mutation affects the plasmid's function.