Opher Gileadi Lab:
TREAT-AD plasmids 2021
Gileadi, et al
unpublished
Gileadi, et al
unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175448 | CD44I-c001 | Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172. |
| 175449 | CD44I-c011 | Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography. |
| 175450 | CD44I-c013 | Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220. |
| 175451 | CD44I-c014 | Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220. Can be biotinylated. |
| 175452 | CD44MMA-c002 | Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, Q23-D172. Used for crystallography. |
| 175453 | CD44U-c010 | Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. |
| 175454 | CD44U-c011 | Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated. |
| 175455 | CD44Z-c011 | Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649. |
| 175456 | CNN3A-c002 | Protein expression in bacterial cells. Entire CDS, M1-Y329. |
| 175457 | CNN3A-c007 | Protein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329. |
| 175458 | EPB41L3A-c000 | Protein expression in bacterial cells. Entire CDS, M1-D1087. |
| 175459 | EPB41L3A-c001 | Protein expression in bacterial cells. FERM domain, M1-L390. |
| 175460 | EPB41L3A-c021 | Protein expression in bacterial cells. FERM domain, P107-L390. Used for crystallography (PDB:6IBE). |
| 175461 | EZRA-c004 | Protein expression in bacterial cells. FERM domain, M1-Q322. |
| 175463 | MDKA-c009 | Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143 |
| 175464 | MDKA-c014 | Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143. Fused to IgG Fc. |
| 175465 | MSNA-c000 | Protein expression in bacterial cells. Entire CDS, M1-M577 |
| 175466 | MSNA-c001 | Protein expression in bacterial cells. FERM domain, M1-E346. Used for crystallography (PDB:6TXQ). |
| 175467 | MSNA-c007 | Protein expression in bacterial cells. FERM domain, M1-D298. Used for NMR studies. |
| 175468 | MSNA-c021 | Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R point mutation that reduces binding to CD44. |
| 175469 | MSNA-c023 | Protein expression in bacterial cells. FERM domain, M1-E346. Can be biotinylated. |
| 175470 | MSNA-c028 | Protein expression in bacterial cells. FERM domain, M1-E346. Contains H288A point mutation that reduces binding to CD44. |
| 175471 | MSNA-c029 | Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44. |
| 175472 | NF2A-c001 | Protein expression in bacterial cells. FERM domain, M1-R338. |
| 175473 | PRDX1A-c001 | Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated. |
| 175474 | PRDX6A-c001 | Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated. |
| 175475 | RAB5AA-c003 | Protein expression in bacterial cells. Entire CDS, M1-N215. |
| 175476 | RAB5AA-c006 | Protein expression in bacterial cells. GTPase domain, G15-N184. Can be used for crystallography. |
| 175477 | RABEP1A-c019 | Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised. |
| 175478 | RABGEF1A-c014 | Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised. |
| 175479 | RDXB-c004 | Protein expression in bacterial cells. FERM domain, M1-A321. |
| 175480 | SDCBPA-c000 | Protein expression in bacterial cells. Entire CDS, M1-V288. |
| 175482 | SDCBPA-c010 | Protein expression in bacterial cells. PDZ1 and PDZ2 domains, A106-V288. Can be used for crystallography. |
| 175483 | SDCBPA-c011 | Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), A106-F275. |
| 175484 | SDCBPA-c020 | Protein expression in bacterial cells. PDZ1 and PDZ2 domains, G111-V288. |
| 175485 | SDCBPA-c021 | Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), G111-F275. |
| 175486 | SNX32A-c014 | Protein expression in bacterial cells. PX domain, S17-M197 |
| 175487 | STX4AA-c005 | Protein expression in bacterial cells. Coiled-coil domains, M1-L275. |
| 175488 | SYKA-c014 | Protein expression in insect cells. Entire CDS, M1-N635. Codon optimised. |
| 175489 | SYKA-c020 | Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography. |
| 175490 | SYKA-c022 | Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated. |
| 175491 | XX01CAPN1A-c001 | Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised. |
| 175492 | XX01CAPN2A-c001 | Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised. |
| 175493 | YSCD44A-c002 | Protein expression in bacterial cells. Single chain Fv antibody to human CD44 Link domain. Can be used for crystallography. |