pAAV-hAdipoq-GFP
(Plasmid #176215)

• Purpose: Expresses GFP under the control of distal enhancer and the promoter of human adiponectin gene
• Depositing Lab: Yu-Hua Tseng
• Publication: Shamsi et al Nat Commun. 2020 Mar 17;11(1):1421. doi: 10.1038/s41467-020-15055-9.

Backbone

• Vector backbone: pAAV-CBA-w
• Backbone size w/o insert (bp): 5050
• Total vector size (bp): 5773
• Modifications to backbone: The CBA promoter in the pAAV-CBA-W backbone was replaced with the human Adiponectin enhancer and promoter sequences.
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Insert Size (bp): 723

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: AACTACTCGAGaccatggtgagcaagggcga
• 3′ sequencing primer: TATTCAGCGGCCGCTTACTTGTACAGCTCGTCCATGC

Resource Information

• Supplemental Documents:
  • paav-hadipoq-gfp.gb
  • Cloning protocol
• A portion of this plasmid was derived from a plasmid made by: The Gfp cDNA was derived from pLenti-GFP-Blast (Addgene, plasmid #17445).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry