|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17628||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral
Selectable markersHygromycin ; GFP
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTetO7 - UBC promoter - GFP - PGK promoter - Hygro
- Cloning method Restriction Enzyme
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Lentiviral dual promoter/dual-gene expression vector is dubbed Duet (for Dual-gene, excisable transgene).
Tet-Inducible Vector. The 309bp tetO7 sequence was PCR-amplified from the template pLVTH-siGFP. The inducible iDuet011A vector has the tetO7 sequence inserted at the PacI site (with a BstXI linker), immediately upstream of the UBC promoter
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:iDuet011a was a gift from Linzhao Cheng (Addgene plasmid # 17628 ; http://n2t.net/addgene:17628 ; RRID:Addgene_17628)
For your References section:Inducible and reversible transgene expression in human stem cells after efficient and stable gene transfer. Zhou BY, Ye Z, Chen G, Gao ZP, Zhang YA, Cheng L. Stem Cells. 2007 Mar . 25(3):779-89. 10.1634/stemcells.2006-0128 PubMed 17158240
Map uploaded by the depositor.