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AiP5001 RabV-CVS-N2cdeltaG-histone-EGFP
(Plasmid #176283)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 176283 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 176283-D50 50 μg of DNA in Tris buffer $495
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DNA 176283-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    Rabies CVS N2c(deltaG)
  • Backbone manufacturer
    Matthias J. Schnell, Thomas Jefferson University
  • Backbone size w/o insert (bp) 14061
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1116
  • Entrez Gene
    H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note there are the following mutations in RabV- G1570S, R1628H, and V1630A. Depositor confirms plasmid is functional.

Please visit https://www.biorxiv.org/content/10.1101/2021.09.29.459010v1.abstract for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AiP5001 RabV-CVS-N2cdeltaG-histone-EGFP was a gift from Allen Institute for Brain Science & Shenqin Yao (Addgene plasmid # 176283 ; http://n2t.net/addgene:176283 ; RRID:Addgene_176283)

  • For your References section:

    A whole-brain monosynaptic input connectome to neuron classes in mouse visual cortex. Yao S, Wang Q, Hirokawa KE, Ouellette B, Ahmed R, Bomben J, Brouner K, Casal L, Caldejon S, Cho A, Dotson NI, Daigle TL, Egdorf T, Enstrom R, Gary A, Gelfand E, Gorham M, Griffin F, Gu H, Hancock N, Howard R, Kuan L, Lambert S, Lee EK, Luviano J, Mace K, Maxwell M, Mortrud MT, Naeemi M, Nayan C, Ngo NK, Nguyen T, North K, Ransford S, Ruiz A, Seid S, Swapp J, Taormina MJ, Wakeman W, Zhou T, Nicovich PR, Williford A, Potekhina L, McGraw M, Ng L, Groblewski PA, Tasic B, Mihalas S, Harris JA, Cetin A, Zeng H. Nat Neurosci. 2023 Feb;26(2):350-364. doi: 10.1038/s41593-022-01219-x. Epub 2022 Dec 22. 10.1038/s41593-022-01219-x PubMed 36550293
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