pGST2-Ub CPS (8-16)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17631||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneparallel GST2
- Backbone size w/o insert (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameUb CPS (8-16)
SpeciesS. cerevisiae (budding yeast)
Entrez GeneRPL40A (a.k.a. YIL148W, CEP52A, UB11, UBI1)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Terms and Licenses
The vector backbone, parallel GST2, was made by replacing the polylinker of pGEX4T1 with the polylinker isolated from the baculovirus expression plasmid pFastBac (see PMID: 10024467).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGST2-Ub CPS (8-16) was a gift from James Hurley (Addgene plasmid # 17631 ; http://n2t.net/addgene:17631 ; RRID:Addgene_17631)
For your References section:Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5. Lee S, Tsai YC, Mattera R, Smith WJ, Kostelansky MS, Weissman AM, Bonifacino JS, Hurley JH. Nat Struct Mol Biol. 2006 Mar . 13(3):264-71. 10.1038/nsmb1064 PubMed 16462746