pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB
(Plasmid
#176587)
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PurposeBacterial expression of His14-MBP-TEV-AmaTnpB
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET45b(+)
- Backbone size w/o insert (bp) 5062
- Total vector size (bp) 7390
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameMBP tag
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SpeciesE. coli
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Insert Size (bp)1098
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GenBank IDWP_040064394.1
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Tag
/ Fusion Protein
- His14 tag (N terminal on backbone)
Gene/Insert 2
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Gene/Insert nameTnpB
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SpeciesAlicyclobacillus macrosporangiidus
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Insert Size (bp)1122
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GenBank IDWP_074954139.1
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB was a gift from Feng Zhang (Addgene plasmid # 176587 ; http://n2t.net/addgene:176587 ; RRID:Addgene_176587) -
For your References section:
The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases. Altae-Tran H, Kannan S, Demircioglu FE, Oshiro R, Nety SP, McKay LJ, Dlakic M, Inskeep WP, Makarova KS, Macrae RK, Koonin EV, Zhang F. Science. 2021 Oct;374(6563):57-65. doi: 10.1126/science.abj6856. Epub 2021 Sep 9. 10.1126/science.abj6856 PubMed 34591643
