pCSJ982
(Plasmid #176622)

• Purpose: Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
• Depositing Lab: Alexander Varshavsky
• Publication: Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;117(25):14158-14167. doi: 10.1073/pnas.2007085117. Epub 2020 Jun 8.

Backbone

• Vector backbone: pJO629
• Backbone manufacturer: Alexander Varshavsky's lab
• Backbone size w/o insert (bp): 8017
• Total vector size (bp): 9328
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ub-MPGLW-Fbp1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1355
• Mutation: Changed first 4 N-terminal amino acid residues of Fbp1 to PGLW
• Promoter: TDH3tc3
• Tag / Fusion Protein:
  • Ubiquitin N-terminal fusion and triple flag tag C-terminal fusion

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: M13-forward
• 3′ sequencing primer: M13-reverse

Resource Information

• Supplemental Documents:
  • pCSJ982-sequence.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry