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Recognition of nonproline N-terminal residues by the Pro/N-degron pathway.
Dong C, Chen SJ, Melnykov A, Weirich S, Sun K, Jeltsch A, Varshavsky A, Min J
Proc Natl Acad Sci U S A. 2020 Jun 23;117(25):14158-14167. doi: 10.1073/pnas.2007085117. Epub 2020 Jun 8. PubMed Article

Plasmids from Article

ID Plasmid Purpose
176622pCSJ982Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Antibodies from Article