pEE060
(Plasmid #176817)

• Purpose: Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression
• Depositing Lab: Gregg Beckham
• Publication: Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.

Backbone

• Vector backbone: pET21b(+)
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5366
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 611
• Alt name: Saccharopolyspora flava WP_093412886.1
• Species: Saccharopolyspora flava
• Insert Size (bp): 780
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7 For
• 3′ sequencing primer: T7 Rev

Resource Information

• Supplemental Documents:
  • pEE060.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Accession Number WP_093412886.1