Skip to main content
Addgene

pDL52 (clone76)
(Plasmid #188579)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188579 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5021
  • Total vector size (bp) 13300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For cloning DH5alpha is fine. For expression, E. coli BL21(DE3) is necessary.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ribA
  • Species
    E. coli
  • Mutation
    Codon optimization, synthetic ribosome binding sites
  • Promoter T7 variants

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    ribD
  • Species
    E. coli
  • Mutation
    Codon optimization, synthetic ribosome binding sites

Gene/Insert 3

  • Gene/Insert name
    yigB
  • Species
    E. coli
  • Mutation
    Codon optimization, synthetic ribosome binding sites

Gene/Insert 4

  • Gene/Insert name
    fbiC
  • Species
    Mycetohabitans rhizoxinica
  • Mutation
    Codon optimization, synthetic ribosome binding sites

Gene/Insert 5

  • Gene/Insert name
    cofC
  • Species
    Methanocaldococus jannaschii
  • Mutation
    Codon optimization, synthetic ribosome binding sites

Gene/Insert 6

  • Gene/Insert name
    cofD
  • Species
    Methanocaldococus jannaschii
  • Mutation
    Codon optimization, synthetic ribosome binding sites

Gene/Insert 7

  • Gene/Insert name
    cofI
  • Species
    Thermomicrobium roseum
  • Mutation
    Codon optimization, synthetic ribosome binding sites

Gene/Insert 8

  • Gene/Insert name
    cofE
  • Species
    Methanocaldococus jannaschii
  • Mutation
    Codon optimization, synthetic ribosome binding sites

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequencing of pDL52 plasmids using primers binding the (repetitive) T7 promotor region is only possible after the separation of the two operons e.g. by restriction digestion with HindIII and NcoI followed by purification of the two fragments from preparative agarose gels.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDL52 (clone76) was a gift from Gerald Lackner (Addgene plasmid # 188579 ; http://n2t.net/addgene:188579 ; RRID:Addgene_188579)
  • For your References section:

    High-yield production of coenzyme F420 in Escherichia coli by fluorescence-based screening of multi-dimensional gene expression space. Last D, Hasan M, Rothenburger L, Braga D, Lackner G. Metab Eng. 2022 Jul 18. pii: S1096-7176(22)00093-3. doi: 10.1016/j.ymben.2022.07.006. 10.1016/j.ymben.2022.07.006 PubMed 35863619