Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pTPTP alpha
(Plasmid #17691)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17691 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTet-splice
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5178
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PTPalpha
  • Alt name
    receptor protein tyrosine phosphatase alpha
  • Alt name
    RPTPalpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2406
  • Entrez Gene
    PTPRA (a.k.a. HEPTP, HLPR, HPTPA, HPTPalpha, LRP, PTPA, PTPRL2, R-PTP-alpha, RPTPA)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer LNCX
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PTPalpha-HA was amplified from plasmid pXJ41PTPalpha using the 5' primer 5'-CGCCAAGCTTGGCCACCATGGATTCCTGGTTCATTCTTGT-3' and the 3' primer 5'-TGTCAAGCTTCATGCGTAGTCTGCACGTCGTATGGGTACTTGAAGT- TGGCATAATCTGA-3'. The 5' primer contains a HindIII site, a Kozak consensus sequence CCACC and the start codon. The 3' primer contains the coding sequence for the HA epitope YPYDVPDYA, the stop codon and a HindIII site. HindIII-cleaved PCR products were ligated into pTet-Splice. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk (Life Technologies), it expresses protein only in the absence of doxycycline.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTPTP alpha was a gift from Xinmin Zheng (Addgene plasmid # 17691 ; http://n2t.net/addgene:17691 ; RRID:Addgene_17691)
  • For your References section:

    A phosphotyrosine displacement mechanism for activation of Src by PTPalpha. Zheng XM, Resnick RJ, Shalloway D. EMBO J. 2000 Mar 1. 19(5):964-78. 10.1093/emboj/19.5.964 PubMed 10698938