|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||176952||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerInvitrogen (V36620)
- Backbone size w/o insert (bp) 5983
Vector typeMammalian Expression
Selectable markersEmGFP fused to Blasticidin (bsd)-resistance marker
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)279
Entrez GeneS100B (a.k.a. NEF, S100, S100-B, S100beta)
- Promoter CMV
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer V5 reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
S100B (NM_006272) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:S100B_pcDNA6.2/EmGFP-Bsd was a gift from Roger Reeves (Addgene plasmid # 176952 ; http://n2t.net/addgene:176952 ; RRID:Addgene_176952)