S100B_pcDNA6.2/EmGFP-Bsd
(Plasmid
#176952)
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PurposeMammalian expression vector encoding S100B and EmGFP-Bsd
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176952 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA6.2/EmGFP-Bsd/V5-DEST
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Backbone manufacturerInvitrogen (V36620)
- Backbone size w/o insert (bp) 5983
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Vector typeMammalian Expression
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Selectable markersEmGFP fused to Blasticidin (bsd)-resistance marker
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameS100B
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Alt nameNEF
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Alt nameS100
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SpeciesH. sapiens (human)
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Insert Size (bp)279
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Entrez GeneS100B (a.k.a. NEF, S100, S100-B, S100beta)
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer V5 reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDerived from Addgene #53895
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
S100B (NM_006272) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
S100B_pcDNA6.2/EmGFP-Bsd was a gift from Roger Reeves (Addgene plasmid # 176952 ; http://n2t.net/addgene:176952 ; RRID:Addgene_176952)
