pBW359
(Plasmid
#177119)
-
PurposeMammalian URT vector -pKP496 SacII-ClaI-flag
-
Depositing Lab
-
Depositing OrganizationCalifornia Institute of Technology
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177119 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 177119-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 177119-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backboneMammalian URT vector
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameRGS4
-
SpeciesH. sapiens (human)
-
GenBank ID
-
Entrez GeneRGS4 (a.k.a. RGP4, SCZD9)
-
Tags
/ Fusion Proteins
- Mammalian URT vector -pKP496 SacII-ClaI-flag
- Mammalian URT vector -pKP496 SacII-ClaI-flag
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 177119-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 177119-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBW359 was a gift from Alexander Varshavsky (Addgene plasmid # 177119 ; http://n2t.net/addgene:177119 ; RRID:Addgene_177119) -
For your References section:
Analyzing N-terminal Arginylation through the Use of Peptide Arrays and Degradation Assays. Wadas B, Piatkov KI, Brower CS, Varshavsky A. J Biol Chem. 2016 Sep 30;291(40):20976-20992. doi: 10.1074/jbc.M116.747956. Epub 2016 Aug 10. 10.1074/jbc.M116.747956 PubMed 27510035
