pHR-IDR1-mCherry-Cry2
(Plasmid #177125)

• Purpose: Express IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay
• Depositing Lab: Wenbo Li
• Publication: Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9.

Backbone

• Vector backbone: Addgene 101221
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YTHDC1 (IDR1 domain)
• Species: H. sapiens (human)
• Mutation: IDR1 domain only
• Entrez Gene: YTHDC1 (a.k.a. YT521, YT521-B)
• Promoter: SFFV
• Tag / Fusion Protein:
  • mCherry-CRY2 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry