pRS42K_PCP-VP64_B-PP7
(Plasmid #177157)

• Purpose: Expresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cells
• Depositing Lab: Gautam Dantas
• Publication: Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-514. doi: 10.1021/acssynbio.1c00384. Epub 2021 Dec 23.

Backbone

• Vector backbone: pRS42K
• Backbone manufacturer: EUROSCARF
• Backbone size w/o insert (bp): 7364
• Total vector size (bp): 7946
• Modifications to backbone: ADH1 promoter and ADH2 terminator were introduced. Another expression cassette of scRNA (A-PP7) was introduced following PCP-VP64 cassette.
• Vector type: Yeast Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: PCP-VP64
• Species: Synthetic
• Insert Size (bp): 582
• Promoter: ADH1 promoter
• Tag / Fusion Protein:
  • SV40 NLS (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Gene/Insert 2

• Gene/Insert name: B-PP7
• Species: Synthetic
• Insert Size (bp): 127
• Promoter: SNR52 promoter

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • prs42k_pcp-vp64_b-pp7.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry