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A Tunable and Expandable Transactivation System in Probiotic Yeast Saccharomyces boulardii.
Kwak S, Mahmud B, Dantas G
ACS Synth Biol. 2022 Jan 21;11(1):508-514. doi: 10.1021/acssynbio.1c00384. Epub 2021 Dec 23. PubMed Article

Plasmids from Article

ID Plasmid Purpose
177155pRS42H_PTEF1_dCas9_TCYC1Expresses dCas9 in yeast cells
177156pRS42K_PCP-VP64_A-PP7Expresses transactivator (PCP-VP64) and scRNA (A-PP7) in yeast cells
177157pRS42K_PCP-VP64_B-PP7Expresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cells
177160pRS42K_MCP-VP64_C-MS2Expresses transactivator (MCP-VP64) and scRNA (C-MS2) in yeast cells
177161pRS42K_MCP-VP64_D-MS2Expresses transactivator (MCP-VP64) and scRNA (D-MS2) in yeast cells
177162pRS426-PGAL7-A_GFP_TCYC1Expresses yeGFP under the control of a synthetic promoter (PGAL7-A)
177163pRS426-PGAL7-B_GFP_TCYC1Expresses yeGFP under the control of a synthetic promoter (PGAL7-B)
177164pRS426-PGAL7-C_RFP_TCYC1Expresses yeRFP under the control of a synthetic promoter (PGAL7-C)
177165pRS426-PGAL7-D_RFP_TCYC1Expresses yeRFP under the control of a synthetic promoter (PGAL7-D)

Antibodies from Article