pRS426-PGAL7-A_GFP_TCYC1
(Plasmid #177162)

• Purpose: Expresses yeGFP under the control of a synthetic promoter (PGAL7-A)
• Depositing Lab: Gautam Dantas
• Publication: Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-514. doi: 10.1021/acssynbio.1c00384. Epub 2021 Dec 23.

Backbone

• Vector backbone: pRS426
• Backbone manufacturer: EUROSCARF
• Backbone size w/o insert (bp): 6091
• Total vector size (bp): 6805
• Modifications to backbone: Synthetic promoter (PGAL7-A) and CYC1 terminator were introduced.w
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: yeGFP
• Species: Synthetic
• Insert Size (bp): 714
• Promoter: Synthetic promoter (PGAL7-A)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • prs426-pgal7-a_gfp_tcyc1.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene #24549

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was used as a template to amplify the reporter cassette for the genomic integration.