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pRK1036
(Plasmid #17728)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17728 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pKM596
  • Backbone manufacturer
    Waugh lab
  • Backbone size w/o insert (bp) 8264
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NusG
  • Alt name
    N-utilization substance G
  • Species
    Aquifex aeolicus
  • Insert Size (bp)
    747
  • GenBank ID
    NC_000918
  • Entrez Gene
    nusG
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • His6 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid produces an MBP-NusG-His6 fusion protein with a recognition site for TVMV protease (ETVRFQS) between MBP and NusG-His6.

See the Notes link from the Plasmid Links box for the amino acid sequence of the MBP-NusG-His fusion protein with TEV site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK1036 was a gift from David Waugh (Addgene plasmid # 17728 ; http://n2t.net/addgene:17728 ; RRID:Addgene_17728)
  • For your References section:

    Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro. Nallamsetty S, Kapust RB, Tozser J, Cherry S, Tropea JE, Copeland TD, Waugh DS. Protein Expr Purif. 2004 Nov . 38(1):108-15. 10.1016/j.pep.2004.08.016 PubMed 15477088