pF1-Hs Cdk1/cyclin B1
(Plasmid #177330)

• Purpose: Co-expression of the human active Cdk1/cyclin B1 kinase complex
• Depositing Lab: Andreas Boland
• Publication: Yu et al Nature. 2021 Aug;596(7870):138-142. doi: 10.1038/s41586-021-03764-0. Epub 2021 Jul 21.

Backbone

• Vector backbone: pF1
• Backbone size w/o insert (bp): 5334
• Total vector size (bp): 7524
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Cyclin-dependent kinase 1
• Alt name: CDK1
• Alt name: CDC2
• Species: H. sapiens (human)
• Insert Size (bp): 891
• GenBank ID: CAA28963.1
• Entrez Gene: CDK1 (a.k.a. CDC2, CDC28A, P34CDC2)
• Tag / Fusion Protein:
  • 8 x His (C terminal on backbone)

Gene/Insert 2

• Gene/Insert name: G2/mitotic-specific cyclin-B1
• Alt name: CCNB1
• Alt name: cyclin B1
• Species: H. sapiens (human)
• Insert Size (bp): 1299
• GenBank ID: BAF82120.1
• Entrez Gene: CCNB1 (a.k.a. CCNB)
• Tag / Fusion Protein:
  • 2 x Strep-tag (C terminal on backbone)

Resource Information

• Supplemental Documents:
  • pF1_CDK1:cyclin B1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Genes are gene-optimised for insect cell expression and have been ordered from GeneArts.