pSSa13
(Plasmid
#177371)
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PurposeExpresses red-shifted luciferase under ScPGK1 promoter in Candida glabrata
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Depositing Lab
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Depositing OrganizationUniversite de Lausanne (UNIL)
Located in Switzerland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177371 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 177371-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 177371-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepVS20
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Selectable markersSAT1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCgLUCopt-mut2
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Insert Size (bp)1653
- Promoter ScPGK1p
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer GCCAAGCGCGCAATTAAC
- 3′ sequencing primer CCTTCTTAATGTTCTTAG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 177371-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 177371-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSSa13 was a gift from Dominique Sanglard (Addgene plasmid # 177371 ; http://n2t.net/addgene:177371 ; RRID:Addgene_177371) -
For your References section:
Investigating Candida glabrata Urinary Tract Infections (UTIs) in Mice Using Bioluminescence Imaging. Schrevens S, Sanglard D. J Fungi (Basel). 2021 Oct 9;7(10). pii: jof7100844. doi: 10.3390/jof7100844. 10.3390/jof7100844 PubMed 34682265
Map uploaded by the depositor.
