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CJ40 (let-7 seed mutant perfect site)
(Plasmid #17773)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 17773 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    David Sabatini Lab
  • Vector type
    Insect Expression, Luciferase ; Drosophila metallothionein gene promoter drives expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    let-7 seed mutant perfect site
  • Alt name
  • Species
    D. melanogaster (fly)
  • Mutation
    seed mutant
  • Entrez Gene
    let-7 (a.k.a. Dmel_CR32968, CR32968, Dmel\CR32968, Let-7, dme-let-7, let7)
  • Tag / Fusion Protein
    • renilla luciferase (N terminal on insert)

Cloning Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): let-7-psm (gagctcACTATACAACCTACAAGCACAactagt). Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CJ40 (let-7 seed mutant perfect site) was a gift from David Bartel (Addgene plasmid # 17773 ; ; RRID:Addgene_17773)
  • For your References section:

    Intronic microRNA precursors that bypass Drosha processing. Ruby JG, Jan CH, Bartel DP. Nature. 2007 Jul 5. 448(7149):83-6. 10.1038/nature05983 PubMed 17589500