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CJ64 (miR-1003 5' splice site mut)
(Plasmid #17785)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 17785 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pCaSpeR4-tubulin promoter
  • Backbone size w/o insert (bp) 10200
  • Vector type
    Drosophila P-element vector

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    dme-miR-1003 minigene 5' splice mut
  • Alt name
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
  • Mutation
    5' splice site mut + compsensatory stem

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Site-directed mutagenesis was used to make 5' splice site mutations with the indicated primers: GCTGCGCAGA ACGTGGGCAT CTGGATGTGG TTGGC and GCCAACCACA TCCAGATGCC CACGTTCTGC GCAGC; CCTCTCACAT TTACATGTTC ACAGGCGCCG TGAG and CTCACGGCGC CTGTGAACAT GTAAATGTGA GAGG. pCaSpeR4 contains a ~2.4 kb tubulin promoter inserted between the EcoRI and KpnI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CJ64 (miR-1003 5' splice site mut) was a gift from David Bartel (Addgene plasmid # 17785 ; ; RRID:Addgene_17785)
  • For your References section:

    Intronic microRNA precursors that bypass Drosha processing. Ruby JG, Jan CH, Bartel DP. Nature. 2007 Jul 5. 448(7149):83-6. 10.1038/nature05983 PubMed 17589500