pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK
(Plasmid #177868)

• Purpose: Cloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).
• Depositing Lab: Gil Westmeyer
• Publication: Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. doi: 10.1038/s41556-021-00678-x. Epub 2021 Jun 3.

Backbone

• Vector backbone: pBlueScript-KS II (+)
• Vector type: Mammalian Expression, CRISPR, Synthetic Biology, Unspecified
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mGreenLantern-based EXSISERS
• Species: H. sapiens (human), S. cerevisiae (budding yeast), Synthetic
• Tag / Fusion Protein:
  • OLLAS

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • GA886_pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HMGU has filed a patent (WO2020161236A1 “Detection of splice events of a gene of interest”) disclosing the capabilities of EXSISERS, and retains ownership rights to EXSISERS Material.