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Addgene

p2xFLAGhYAP2-S127A Notes

Addgene Diagnostic Digest

This digest shows the NEB 1kb ladder, the uncut plasmid and the plasmid digested with the enzyme EcoRI.

The digest did not go to completion but the 2 bands that represent digested plasmid match the size of the backbone (~4.7kb) and the size of the insert (~1.6kb). We see the expected pattern.