Psicheck Cdk2ap1Exon1
(Plasmid
#178028)
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Purposeexpression vector to test translation efficiency by luciferase reporter.
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Depositing Lab
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Depositing OrganizationUniversity of California, Berkeley
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178028 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 178028-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 178028-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepsiCHECK-2
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 6273
- Total vector size (bp) 6251
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Modifications to backboneNone
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Vector typeMammalian Expression, Luciferase ; Nonviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCdk2ap1
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Alt nameCdk2ap1 Canonical
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SpeciesM. musculus (mouse)
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Insert Size (bp)257
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GenBank ID13445 13445
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Entrez GeneCdk2ap1 (a.k.a. Apc10, Cdkap1, DORC1, Doc1, ST19, doc-1, p12)
- Promoter Cloned in
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Tag
/ Fusion Protein
- Luciferase (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TTAATACGACTCACTATAGGgcggccgcccctgcgccgcg
- 3′ sequencing primer TCGTACACCTTGGAAGCCATccccgcgggcggcgggcgcg
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 178028-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 178028-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Psicheck Cdk2ap1Exon1 was a gift from Lin He (Addgene plasmid # 178028 ; http://n2t.net/addgene:178028 ; RRID:Addgene_178028) -
For your References section:
A mouse-specific retrotransposon drives a conserved Cdk2ap1 isoform essential for development. Modzelewski AJ, Shao W, Chen J, Lee A, Qi X, Noon M, Tjokro K, Sales G, Biton A, Anand A, Speed TP, Xuan Z, Wang T, Risso D, He L. Cell. 2021 Oct 7. pii: S0092-8674(21)01104-1. doi: 10.1016/j.cell.2021.09.021. 10.1016/j.cell.2021.09.021 PubMed 34644528
Map uploaded by the depositor.
