pUltra-5HTP
(Plasmid
#178042)
-
PurposeExpress modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178042 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepUltra
- Backbone size w/o insert (bp) 4067
- Total vector size (bp) 5362
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert 1
-
Gene/Insert nameModified ScTrpRS for 5HTP incoporation
-
SpeciesS. cerevisiae (budding yeast)
-
Insert Size (bp)1299
-
MutationT107C, P254T, C255A
Gene/Insert 2
-
Gene/Insert namesuppressor tRNA for 5HTP incorporation
-
SpeciesS. cerevisiae (budding yeast)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pUltra-5HTP was a gift from Han Xiao (Addgene plasmid # 178042 ; http://n2t.net/addgene:178042 ; RRID:Addgene_178042) -
For your References section:
Creation of Bacterial cells with 5-Hydroxytryptophan as a 21(st) Amino Acid Building Block. Chen Y, Tang J, Wang L, Tian Z, Cardenas A, Fang X, Chatterjee A, Xiao H. Chem. 2020 Oct 8;6(10):2717-2727. doi: 10.1016/j.chempr.2020.07.013. Epub 2020 Aug 12. 10.1016/j.chempr.2020.07.013 PubMed 33102928
Map uploaded by the depositor.
