pACEBac1-beta-actin
(Plasmid
#178076)
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PurposeInsect cell expression of beta-actin
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178076 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepACEBac1
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Backbone manufacturerGeneva Biotech
- Total vector size (bp) 4022
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namebeta-actin
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SpeciesH. sapiens (human)
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Insert Size (bp)1125
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Entrez GeneACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
Resource Information
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A portion of this plasmid was derived from a plasmid made byA plasmid encoding human β-actin was a kind gift from Dr. Gregory Alushin (The Rockefeller University, New York, NY)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACEBac1-beta-actin was a gift from Tarun Kapoor (Addgene plasmid # 178076 ; http://n2t.net/addgene:178076 ; RRID:Addgene_178076) -
For your References section:
Biochemical reconstitutions reveal principles of human gamma-TuRC assembly and function. Wieczorek M, Ti SC, Urnavicius L, Molloy KR, Aher A, Chait BT, Kapoor TM. J Cell Biol. 2021 Mar 1;220(3). pii: 211719. doi: 10.1083/jcb.202009146. 10.1083/jcb.202009146 PubMed 33496729
