pANG01
(Plasmid #178083)

• Purpose: (Empty Backbone) Carbenicillin resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG02.
• Depositing Lab: Sören Abel
• Publication: Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.1016/j.plasmid.2021.102608. Epub 2021 Nov 18.

Backbone

• Vector backbone: pGP704, pBAD33, pDONR201
• Backbone manufacturer: NA, NA, Invitrogen
• Modifications to backbone: pANG01 was constructed with the bla from pGP704, oriTRP4 from pG704, and the oriVp15a from pBAD33. The Gateway sequence was obtained from commercial vector pDONR201 (Invitrogen) and inserted into the EcorV sites of minimal vectors by Gibson assembly to include unique PmeI and PacI restriction sites flanking the att sites.
• Vector type: Bacterial Expression
• Promoter: NA

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Plasmid contains ccdB toxin. Strain grows under standard laboratory conditions in LB media supplemented with antibiotics to maintain plasmid.
• Copy number: Low Copy

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 Forward for Gateway site
• 3′ sequencing primer: M13 Reverse for Gateway site

Resource Information

• Supplemental Documents:
  • pANG01.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmids from this article are also used with plasmid pXint129 (www.addgene.org/178208).