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A single step three-strain in vivo Gateway reaction.
Gillman AN, Helleux A, Abel S
Plasmid. 2021 Nov;118:102608. doi: 10.1016/j.plasmid.2021.102608. Epub 2021 Nov 18. PubMed Article

Plasmids from Article

ID Plasmid Purpose
178083pANG01Carbenicillin resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG02.
178084pANG02Gentamycin resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG01 or pANG03.
178085pANG03Tetracycline resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG02.
178086pMARODerivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.

Antibodies from Article