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Purpose(Empty Backbone) An improved vector for BacMam system
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178203 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepFastBac1
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Backbone manufacturerThermofisher
- Backbone size (bp) 4775
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Modifications to backboneadding multiple elements for protein expression in mammalian cells
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Vector typeMammalian Expression
- Promoter CMV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer ggtcttactgacatc
- 3′ sequencing primer ccagtcaatctttcac (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.10.18.464913v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBMCL1 was a gift from Lei Chen (Addgene plasmid # 178203 ; http://n2t.net/addgene:178203 ; RRID:Addgene_178203) -
For your References section:
A co-expression vector for baculovirus-mediated protein expression in mammalian cells. Guo W, Wang M, Chen L. Biochem Biophys Res Commun. 2022 Feb 26;594:69-73. doi: 10.1016/j.bbrc.2022.01.056. Epub 2022 Jan 19. 10.1016/j.bbrc.2022.01.056 PubMed 35074588
Map uploaded by the depositor.
