|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17846||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2000
Entrez GeneDYRK1B (a.k.a. AOMS3, MIRK)
/ Fusion Proteins
- 6xHis (N terminal on backbone)
- Anti-XPress (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The insert is an StuI fragment of the mirk/dyrk1b gene. The EcoRV site was destroyed during cloning, but a new BamHI site was generated at the 3' end.
This plasmid was named pHX9F in the original paper. "After EcoRI digestion, mirk cDNA was ligated into pcDNA3.1/HisA, yielding the hexahistidine fusion plasmid pHX9."
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA-Mirk (pHX9F) was a gift from Eileen Friedman (Addgene plasmid # 17846 ; http://n2t.net/addgene:17846 ; RRID:Addgene_17846)
For your References section:Mirk protein kinase is a mitogen-activated protein kinase substrate that mediates survival of colon cancer cells. Lee K, Deng X, Friedman E. Cancer Res. 2000 Jul 1. 60(13):3631-7. PubMed 10910078