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pcDNA-Mirk (pHX9F)
(Plasmid #17846)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17846 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1/HisA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mirk
  • Alt name
    Dyrk1B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • GenBank ID
    AAF15893
  • Entrez Gene
    DYRK1B (a.k.a. AOMS3, MIRK)
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • Anti-XPress (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The insert is an StuI fragment of the mirk/dyrk1b gene. The EcoRV site was destroyed during cloning, but a new BamHI site was generated at the 3' end.

This plasmid was named pHX9F in the original paper. "After EcoRI digestion, mirk cDNA was ligated into pcDNA3.1/HisA, yielding the hexahistidine fusion plasmid pHX9."

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-Mirk (pHX9F) was a gift from Eileen Friedman (Addgene plasmid # 17846 ; http://n2t.net/addgene:17846 ; RRID:Addgene_17846)
  • For your References section:

    Mirk protein kinase is a mitogen-activated protein kinase substrate that mediates survival of colon cancer cells. Lee K, Deng X, Friedman E. Cancer Res. 2000 Jul 1. 60(13):3631-7. PubMed 10910078