pCMMP-MCS-IRES-eGFP-P2A-iCas9
(Plasmid
#178533)
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PurposeFor genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178533 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMMP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameiCaspase9
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SpeciesH. sapiens (human)
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MutationFKBP12 F36 to V Truncated Caspase9 (135-416; lacking pro-domain)
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Entrez GeneCASP9 (a.k.a. APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains F64L and V163A mutations in EGFP. These mutations are not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMMP-MCS-IRES-eGFP-P2A-iCas9 was a gift from Ryoji Yao (Addgene plasmid # 178533 ; http://n2t.net/addgene:178533 ; RRID:Addgene_178533) -
For your References section:
A protocol for efficient CRISPR-Cas9-mediated knock-in in colorectal cancer patient-derived organoids. Okamoto T, Natsume Y, Yamanaka H, Fukuda M, Yao R. STAR Protoc. 2021 Sep 16;2(4):100780. doi: 10.1016/j.xpro.2021.100780. eCollection 2021 Dec 17. 10.1016/j.xpro.2021.100780 PubMed 34585151
