pLX_HA
(Plasmid #178534)

• Purpose: (Empty Backbone) Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)
• Depositing Lab: William Hahn
• Publication: Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(21)00488-9. doi: 10.1016/j.cels.2021.12.005.

Backbone

• Vector backbone: pLX_TRC317 (https://portals.broadinstitute.org/gpp/public/vector/details?vector=pLX_TRC317)
• Backbone size (bp): 8409
• Modifications to backbone: c-term HA on backbone instead of V5
• Vector type: Mammalian Expression
• Promoter: EF1a
• Selectable markers: Puromycin
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Cloning Information

• 5′ sequencing primer: EF1a-F (TCAAGCCTCAGACAGTGGTTC)
• 3′ sequencing primer: WPRE-R (CATAGCGTAAAAGGAGCAACA)

Resource Information

• Supplemental Documents:
  • pLX_HA_actual.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry