|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17873||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3300
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameBAF 190a
SpeciesH. sapiens (human)
Entrez GeneSMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI/SalI (destroyed during cloning)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pcDL-F (Common Sequencing Primers)
This plasmid is an expression vector for the full length BRG/BAF190 protein which is similar to Drosphila Brm and contains the conserved ATPase domain in the yeast SWI2/SNF2 protein.
Addgene QC NGS analysis identifies an R1608Q mutation relative to NP_001122316.1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBJ5 hBRG1 was a gift from Jerry Crabtree (Addgene plasmid # 17873 ; http://n2t.net/addgene:17873 ; RRID:Addgene_17873)
For your References section:BRG1 contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription. Khavari PA, Peterson CL, Tamkun JW, Mendel DB, Crabtree GR. Nature. 1993 Nov 11. 366(6451):170-4. 10.1038/366170a0 PubMed 8232556