psiCHECK2-mMOV10-3'UTR
(Plasmid #178905)

• Purpose: dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase
• Depositing Lab: Constance Ciaudo
• Publication: Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15252/embr.202154458.

Backbone

• Vector backbone: psiCHECK-2
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3560
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: The plasmid was prepped in the HST08 strain for the original study
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3'UTR mMov10
• Species: M. musculus (mouse)
• Insert Size (bp): 383
• Entrez Gene: Mov10 (a.k.a. C77703, Mov-10)
• Promoter: SV40 promoter

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: GGTTCTTTTCCAACGCTAT
• 3′ sequencing primer: CGAGGTCCGAAGACTCATT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2021.11.09.467897 for bioRxiv preprint.