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Sequestration of LINE-1 in cytosolic aggregates by MOV10 restricts retrotransposition.
Arora R, Bodak M, Penouty L, Hackman C, Ciaudo C
EMBO Rep. 2022 Jul 20:e54458. doi: 10.15252/embr.202154458.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
73542pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3Expression of sgRNA targeting LINE-1 and TagBFP
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73543pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1Expression of sgRNA targeting LINE-1 and TagBFP
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178905psiCHECK2-mMOV10-3'UTRdual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase
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178906psiCHECK2-mMOV10-3'UTR-MRE16-mut1dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase
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178907pcDNA3-T11HA-hMOV10-WTexpression of WT hMOV10
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178908pcDNA3-T11HA-hMOV10-CDexpression of catalytic dead hMOV10 K530A
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178909psiCHECK2-mMOV10-3'UTR-MRE153-mut4dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase
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178910psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mutdual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase
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185052T10-T7-hMOV10expression of WT human MOV10
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185053T11-HA-hORF1expression of human L1 ORF1 protein
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Antibodies from Article